New Biomarkers in the Diagnosis of COVID-19: Amino Acids




COVID-19, Metabolomics, Amino Acid profiling, Biomarker, MetaboAnalyst


Background: Coronavirus disease 2019 (Covid-19) is still spreading rapidly around the world and early detection before its severe symptoms begin is essential but remains challenging. There is also very little data on the effects of covid-19 on amino acid metabolism. The aim of this study is to analyze the free amino acid profile in the blood serum of covid-19 patients and to determine their biomarker potential for diagnosis.

Materials and Methods: In this study, blood samples were taken from 30 covid-19 patients and 30 healthy individuals and their amino acid profile was examined by LC-MS/MS. Biomarker and pathway analyzes from multivariate statistical analyzes were performed with the MetaboAnalyst program.

Results: Among the amino acids we analyzed, 6 of them were amino acids with strong potential to be used in the diagnosis of covid-19. According to the AUC value(>0.800), these amino acids are alpha-aminoadipic acid, arginine, proline, alanine, sarcosine and citrulline, respectively. In addition, according to the pathway analysis, it was learned that arginine and proline metabolisms were significantly impaired in covid-19 disease (p<0.001 and impact value: 0.356).

Conclusions: In this study, changes in amino acids in covid-19 patients showed that they may be important in diagnosis, treatment and prognosis. Alpha-aminoadipic acid may be an excellent candidate for diagnosis. A diet rich in depleted amino acids, especially arginine and proline amino acids, may show successful results in the treatment.





How to Cite

AKMEŞE ŞÜKRÜ, KOYUNCU İsmail, Seyhanli ES. New Biomarkers in the Diagnosis of COVID-19: Amino Acids. İJCMBS [Internet]. 2022 Jul. 22 [cited 2024 Jun. 18];2(2):127-35. Available from: